Sirius Red Total Collagen Detection PLATE Kit

Produktübersicht

• Artikelname: Sirius Red Total Collagen Detection PLATE Kit
• Artikelnummer: CHX-9062P
• Hersteller Artikelnummer: 9062P
• Alternativnummer: CHX-9062P
• Hersteller: Chondrex
• Kategorie: Kits/Assays

Produktbeschreibung

Sirius Red Total Collagen Detection Assay PLATE Kit

Zusatzinformationen

Sirius Red is a unique dye which specifically binds to the [Gly-X-Y]n helical structure on fibrillar collagen (type I to V) and does not discriminate between collagen species and types.

Therefore, Chondrex, Inc. provides a Sirius Red Collagen Detection Assay Plate Kit designed to detect the total collagen content in various samples. This kit uses an ELISA plate for various collagen-containing samples such as tissue specimens, cell culture media, and cultured cells. This protocol is ideal for users who have many samples because the total assay working time is less than 60 minutes and 80 samples can be measured in duplicate. Due to the low level of collagen in cell culture media, additional concentration steps may be necessary.

Concentrating Solution (Cat # CHX-90626) for cell culture media samples is NOT included in this kit.

For determining levels of collagen from individual species or different types of samples, Chondrex, Inc. recommends the Sirius Red Collagen Detection Assay Kit using the tube assay method (Cat # CHX-9062), Type I Collagen Detection ELISA Kits, and the Type II Collagen Detection ELISA Kit. Por convenience, the same sample preparation methods may be used for both the Collagen Detection ELISA Kits and the Sirius Red Collagen Detection Assay Kits.

 

Kit Components

• 1 ml Standard - Bovine Type I Collagen (90621)
• 50 ml Sirius Red Solution (90622)
• 50 ml Washing Solution (90623)
• 30 ml Extraction Solution (90624)
• 20 ml 0.5M Acetic Acid (10X Acetic Acid) (90625)
• Flat-Bottom 96-Well Plate (9026), 8-well strips x 12
• 2 V-bottom 96-Well Plates for Centrifugation (90261)
• 1 Flexible 96-Well Plate for Sample Dilution (90262)
• 96-Well ELISA Plate Sealers (90263)

Produkteigenschaften

• Detektionsbereich: 500 µg/ml-8 µg/ml
• Lagerung: -20°C
• Proben: Culture Media, Solubilized Collagen