FagoFlowEx Kit

Product Overview

• Article Name: FagoFlowEx Kit
• Biozol Catalog Number: EXB-ED7042
• Supplier Catalog Number: ED7042
• Alternative Catalog Number: EXB-ED7042
• Manufacturer: EXBIO
• Category: Kits/Assays
• Application: FC

Product Description

The FagoFlowEx Kit is intended for the examination of phagocytic activity of neutrophil granulocytes by measuring the respiratory (oxidative) burst after their stimulation with E. coli bacteria in human heparinized whole blood using flow cytometry

Additional Information

The FagoFlowEx Kit (CE IVD) is intended for the examination of phagocytic activity of neutrophil granulocytes by measuring the respiratory (oxidative) burst after their stimulation with E. coli bacteria in human heparinized whole blood using flow cytometry.

The product is intended for In Vitro Diagnostic Use.

 

Application

Flow Cytometry

Reactivity

Human

 

Content

ED7042-1 E.coli – 5 vials containing lyophilized E. coli bacteria, 1 vial is intended for stimulation of 20 tubes.
ED7042-2 DHR123 – 5 vials containing lyophilized Dihydrorhodamine 123, 1 vial is intended for staining of 60 tubes.
ED7042-3 Stimulation Control – 5 vials containing lyophilized PMA (Phorbol 12-myristate 13-acetate), 1 vial is intended for stimulation of 20 positive controls.
ED7042-4 Lysing Solution – 15 ml

 

Product Properties

• Storage: 2°C to 8°C

Application Notes

• Application Notes: FC (QC tested)

Images

Content ED7042-1 E.coli – 5 vials containing lyophilized E. coli bacteria, 1 vial is intended for stimulation of 20 tubes. ED7042-2 DHR123 – 5 vials containing lyophilized Dihydrorhodamine 123, 1 vial is intended for staining of 60 tubes. ED7042-3 Stimulation Control – 5 vials containing lyophilized PMA (Phorbol 12-myristate 13-acetate), 1 vial is intended for stimulation of 20 positive controls. ED7042-4 Lysing Solution – 15 ml

Separation of stimulated neutrophil granulocytes (red-filled) from non-stimulated neutrophil granulocytes (negative control; black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood of donor with chronic granulomatous disease (CGD), causing defect of respiratory burst (SI = 11), stained using FagoFlowEx Kit.

Separation of stimulated neutrophil granulocytes (red-filled) from non-stimulated neutrophil granulocytes (negative control; black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood of healthy donor without defect of respiratory burst (SI = 98), stained using FagoFlowEx Kit.

Histograms showing respirátory burst in negative control (left), positive control (middle) and E. coli stimulated sample (right) of human peripheral whole blood of healthy donor without defect of respiratory burst, stained using FagoFlowEx Kit.