This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of
100 µg in 500 µl PBS containing 0.05%BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Reinheit:
Affinity Chromatography
Formulierung:
Purified
Formel:
100 µg in 500 µl PBS containing 0.05%BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Target-Kategorie:
Vimentin (Mesenchymal Cell Marker)
Application Verdünnung:
Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 min
Fig. 3 : Formalin-fixed, para
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