Anti-Sml1 | Suppressor of Mec1 lethality, Polyclonal

Artikelnummer: AGR-AS10-847
Artikelname: Anti-Sml1 | Suppressor of Mec1 lethality, Polyclonal
Artikelnummer: AGR-AS10-847
Hersteller Artikelnummer: AS10-847
Alternativnummer: AGR-AS10-847
Hersteller: Agrisera
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Yeast
Immunogen: KLH-conjugated synthetic peptide derived from known S.cerevisie Sml1 sequence. Gene ID: 854945
Sml1 is a ribonucleotide reductase inhibitor involved in regulation of dNTP production. It is regulated by Mec1p and Rad53p during DNA damage and S phase. Synonymes: Yml058w.
Klonalität: Polyclonal
Molekulargewicht: 11,83 | 11-12 kDa
NCBI: 854945
UniProt: 854945
Reinheit: Serum
Formulierung: Lyophilized
Antibody Type: Polyclonal Antibody
Application Verdünnung: 1 : 1000 (WB)
Anwendungsbeschreibung: Cell preparation for western blot: cells were harvested by centrifugation (4000 x g , 6 min, 4 C), Supernatant was discarded and cells were resuspended in 500 µl cold TCA buffer (20 mM Tris, pH 8, 50 mM ammonium acetate, 2 mM EDTA, 1 tablet/10 ml of Comp