Anti-His-tag | 6xHis (clone HIS,H8 / EH158), IgG2b, Monoclonal

Produktübersicht

• Artikelname: Anti-His-tag | 6xHis (clone HIS,H8 / EH158), IgG2b, Monoclonal
• Artikelnummer: AGR-AS11-1771
• Hersteller Artikelnummer: AS11-1771
• Alternativnummer: AGR-AS11-1771
• Hersteller: Agrisera
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: DOT, ELISA, IP, WB
• Immunogen: KLH-conjugated synthetic peptide 6xHis

Produktbeschreibung

His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzy

Produkteigenschaften

• Klonalität: Monoclonal
• Isotyp: IgG2b
• Reinheit: Total IgG fraction. Protein A purified.
• Formulierung: Liquid
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Application Verdünnung: 1 : 1000 (WB)
• Anwendungsbeschreibung: Antibody is present in 10 mM PBS, pH 7.2His-tag (6,8,10xHis) needs to be properly exposed to allow detection. To prevent target protein folding, extraction should be performed with 6 to 8 M urea or using TCA-acetone precipitation method.