NRT2.1 is a high affinity nitrate transporter which is up-regulated by nitrate. Functions as a repressor of lateral root initiation independently of nitrate uptake. Synonymes: Protein ACH1, Protein LATERAL ROOT INITIATION 1, ACH1, ATNRT2.1, ATNRT2:1,, L
Microsomal fraction is recommended to be used as it will have higher amount of NRT2,1 protein, If a total protein extract is used SDS should be applied from the beginning in extraction buffer as well as protease inhibitor coctail
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