Anti-VIM, Rabbit, Polyclonal

Artikelnummer: ATA-HPA001762
Artikelname: Anti-VIM, Rabbit, Polyclonal
Artikelnummer: ATA-HPA001762
Hersteller Artikelnummer: HPA001762
Alternativnummer: ATA-HPA001762-25,ATA-HPA001762-100
Hersteller: Atlas Antibodies
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IHC, WB
Spezies Reaktivität: Human, Mouse
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Konjugation: Unconjugated
Alternative Synonym: VIM
vimentin
Anti-VIM
Klonalität: Polyclonal
Konzentration: 0.2 mg/ml
Isotyp: IgG
NCBI: 7431
UniProt: P08670
Puffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Reinheit: Affinity purified using the PrEST antigen as affinity ligand
Sequenz: FANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDSLARLDLERKVES
Lagerung: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target-Kategorie: VIM
Antibody Type: Monoclonal Antibody
Application Verdünnung: ICC-IF: 0.25-2 µg/ml, IHC: 1:1000 - 1:2500, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.
Immunohistochemistry analysis in human ovary and skeletal muscle tissues using HPA001762 antibody. Corresponding VIM RNA-seq data are presented for the same tissues.
Immunohistochemical staining of human fallopian tube shows moderate membranous positivity in glandular cells.
Immunohistochemical staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.
Immunohistochemical staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemical staining of human ovary shows moderate cytoplasmic positivity in stromal cells.
Western blot analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
HPA001762
HPA001762
HPA001762