Anti-SIX1, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Anti-SIX1, Rabbit, Polyclonal
• Artikelnummer: ATA-HPA001893
• Hersteller Artikelnummer: HPA001893
• Alternativnummer: ATA-HPA001893-25,ATA-HPA001893-100
• Hersteller: Atlas Antibodies
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
• Konjugation: Unconjugated
• Alternative Synonym: DFNA23

Produktbeschreibung

SIX homeobox 1

Zusatzinformationen

Anti-SIX1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.4 mg/ml
• Isotyp: IgG
• NCBI: 6495
• UniProt: Q15475
• Puffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
• Reinheit: Affinity purified using the PrEST antigen as affinity ligand
• Sequenz: CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS
• Lagerung: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
• Target-Kategorie: SIX1
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Application Verdünnung: ICC-IF: 0.25-2 µg/ml, IHC: 1:500 - 1:1000, WB: 0.04-0.4 µg/ml

Bilder

Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.

Immunohistochemistry analysis in human skeletal muscle and liver tissues using HPA001893 antibody. Corresponding SIX1 RNA-seq data are presented for the same tissues.

Immunohistochemical staining of human salivary gland shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemical staining of human breast cancer shows weak to moderate nuclear positivity in tumor cells.

Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in striated muscle fibers.

Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SIX1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Western blot analysis in mouse cell line NIH-3T3.

Western blot analysis in control (vector only transfected HEK293T lysate) and SIX1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401814).

HPA001893

HPA001893

HPA001893