Anti-KRT8, Rabbit, Polyclonal

Artikelnummer: ATA-HPA049866
Artikelname: Anti-KRT8, Rabbit, Polyclonal
Artikelnummer: ATA-HPA049866
Hersteller Artikelnummer: HPA049866
Alternativnummer: ATA-HPA049866-100
Hersteller: Atlas Antibodies
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IHC, WB
Spezies Reaktivität: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Konjugation: Unconjugated
Alternative Synonym: CARD2, CK8, CYK8, K2C8, K8, KO, Pan-Cancer
keratin 8
Anti-KRT8
Klonalität: Polyclonal
Konzentration: 0.05 mg/ml
Isotyp: IgG
NCBI: 3856
UniProt: P05787
Puffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Reinheit: Affinity purified using the PrEST antigen as affinity ligand
Sequenz: SFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
Lagerung: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target-Kategorie: KRT8
Antibody Type: Monoclonal Antibody
Application Verdünnung: ICC-IF: 0.25-2 µg/ml, IHC: 1:1000 - 1:2500, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.
Western blot analysis in human cell lines MCF-7 and SK-MEL-30 using Anti-KRT8 antibody. Corresponding KRT8 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
Lane 6: Human tonsil tissue
HPA049866
HPA049866
HPA049866