Biotin-14-dCTP

Produktübersicht

• Artikelname: Biotin-14-dCTP
• Artikelnummer: BYT-ORB1733736
• Hersteller Artikelnummer: orb1733736
• Alternativnummer: BYT-ORB1733736-200
• Hersteller: Biorbyt
• Kategorie: Sonstiges

Produktbeschreibung

Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline p

Produkteigenschaften

• Konzentration: 1.0 mM-1.1 mM
• Molekulargewicht: Theoretical MW: 905.78 g/mol (free acid), Detected MW: 905.26 g/mol (free acid)
• Reinheit: 95% (HPLC)
• Formulierung: filtered solution (30 kDa) in 10 mM Tris-HCl, Color: colorless to slightly yellow, pH: 7.5 0.5
• Formel: C31H54N7O16P3S

Anwendungsbeschreibung

• Anwendungsbeschreibung: Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data, Primer Extension with Klenow fragment. Spectroscopic Propertie: lambdamax 272 nm, epsilon 13.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).