MCF-7 Whole Cell Lysate, Unconjugated

Artikelnummer: BYT-ORB348664
Artikelname: MCF-7 Whole Cell Lysate, Unconjugated
Artikelnummer: BYT-ORB348664
Hersteller Artikelnummer: orb348664
Alternativnummer: BYT-ORB348664-500
Hersteller: Biorbyt
Kategorie: Sonstiges
Applikation: SDS-PAGE, WB
Konjugation: Unconjugated
Alternative Synonym: MCF-7 Whole Cell Lysate, MCF7 WCL
MCF-7 Whole Cell Lysate
Konzentration: 1.0 mg/ml
Puffer: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Quelle: Human
Reinheit: The cells were grown in Dulbeccos medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodiu
Formulierung: Liquid
Application Verdünnung: WB: User Optimized
Anwendungsbeschreibung: ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been