HeLa Whole Cell Lysate Doxorubicin Stimulated, Unconjugated

Artikelnummer: BYT-ORB348681
Artikelname: HeLa Whole Cell Lysate Doxorubicin Stimulated, Unconjugated
Artikelnummer: BYT-ORB348681
Hersteller Artikelnummer: orb348681
Alternativnummer: BYT-ORB348681-500
Hersteller: Biorbyt
Kategorie: Sonstiges
Applikation: SDS-PAGE, WB
Konjugation: Unconjugated
Alternative Synonym: HeLa Whole Cell Lysate Doxorubicin Stimulated, HeLa WCL Doxorubicin Stimulated, HeLa Doxorubicin Stimulated Whole Cell Lysate, HeLa Doxorubicin Stimulated WCL
HeLa Whole Cell Lysate Doxorubicin Stimulated
Konzentration: 1.0 mg/ml
Puffer: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Quelle: Human
Reinheit: The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). Cells were treated with 0.5 µg/ml Doxorubicin for 2 h. Cells were washed in PBS and incubated on ice in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris Cl, pH
Formulierung: Liquid (sterile filtered)
Application Verdünnung: WB: User Optimized
Anwendungsbeschreibung: ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been