HeLa Whole Cell Lysate Nocodozole Stimulated, Unconjugated

Produktübersicht

• Artikelname: HeLa Whole Cell Lysate Nocodozole Stimulated, Unconjugated
• Artikelnummer: BYT-ORB348683
• Hersteller Artikelnummer: orb348683
• Alternativnummer: BYT-ORB348683-500
• Hersteller: Biorbyt
• Kategorie: Sonstiges
• Applikation: SDS-PAGE, WB
• Konjugation: Unconjugated
• Alternative Synonym: HeLa Whole Cell Lysate Nocodazole Stimulated, HeLa Nocodazole Stimulated Whole Cell Lysate, HeLa Nocodazole Stimulated WCL, HeLa WCL Nocodazole Stimulated

Produktbeschreibung

HeLa Whole Cell Lysate Nocodozole Stimulated

Produkteigenschaften

• Konzentration: 1.0 mg/ml
• Puffer: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Quelle: Human
• Reinheit: The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). Cells were treated with 3 µg/ml nocodazole for 1 h. Cells were washed in PBS and incubated on ice in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris Cl, pH 7.
• Formulierung: Liquid (sterile filtered)

Anwendungsbeschreibung

• Application Verdünnung: WB: User Optimized
• Anwendungsbeschreibung: ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been