HeLa Cell Nuclear Extract Etoposide Stimulated, Unconjugated

Produktübersicht

• Artikelname: HeLa Cell Nuclear Extract Etoposide Stimulated, Unconjugated
• Artikelnummer: BYT-ORB348685
• Hersteller Artikelnummer: orb348685
• Alternativnummer: BYT-ORB348685-200
• Hersteller: Biorbyt
• Kategorie: Sonstiges
• Applikation: SDS-PAGE, WB
• Konjugation: Unconjugated
• Alternative Synonym: HeLa Cell Nuclear Extract Etoposide Stimulated, HeLa Etoposide Stimulated Nuclear Lysate, HeLa Nuclear Lysate Etoposide Stimulated, HeLa Etoposide Stimulated Nuclear Extract

Produktbeschreibung

HeLa Cell Nuclear Extract Etoposide Stimulated

Produkteigenschaften

• Konzentration: 1.0 mg/ml
• Puffer: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Quelle: Human
• Reinheit: The cells were grown in Eagles medium supplemented with 10% FBS (Fetal Bovine Serum). Cells were treated with 10 µg/ml Etoposide for 13 h. The lysate was prepared by first washing the cells in PBS. Washed cells were then incubated on ice in lysis buffer
• Formulierung: Liquid (sterile filtered)

Anwendungsbeschreibung

• Application Verdünnung: WB: User Optimized
• Anwendungsbeschreibung: ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5