HeLa Cell Nuclear Extract Nocodozole Stimulated, Unconjugated

Artikelnummer: BYT-ORB348686
Artikelname: HeLa Cell Nuclear Extract Nocodozole Stimulated, Unconjugated
Artikelnummer: BYT-ORB348686
Hersteller Artikelnummer: orb348686
Alternativnummer: BYT-ORB348686-200
Hersteller: Biorbyt
Kategorie: Sonstiges
Applikation: SDS-PAGE, WB
Konjugation: Unconjugated
Alternative Synonym: HeLa Cell Nuclear Extract Nocodazole Stimulated, HeLa Nocodazole Stimulated Nuclear Lysate, HeLa Nuclear Lysate Nocodazole Stimulated, HeLa Nocodazole Stimulated Nuclear Extract
HeLa Cell Nuclear Extract Nocodozole Stimulated
Konzentration: 1.0 mg/ml
Puffer: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Quelle: Human
Reinheit: The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). Cells were treated with 0.2 µg/ml Nocodazole for 30 min. The lysate was prepared by first washing the cells in PBS. Washed cells were then incubated on ice in lysis buffer conta
Formulierung: Liquid (sterile filtered)
Application Verdünnung: WB: User Optimized
Anwendungsbeschreibung: ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5