MCF-7 Cell Nuclear Extract, Unconjugated

Produktübersicht

• Artikelname: MCF-7 Cell Nuclear Extract, Unconjugated
• Artikelnummer: BYT-ORB348687
• Hersteller Artikelnummer: orb348687
• Alternativnummer: BYT-ORB348687-200
• Hersteller: Biorbyt
• Kategorie: Sonstiges
• Applikation: WB
• Konjugation: Unconjugated
• Alternative Synonym: MCF-7 Cell Nuclear Extract, MCF7 Cell Nuclear Extract, MCF-7 Nuclear Lysate, MCF7 Lysate Nuclear Extract

Produktbeschreibung

MCF-7 Cell Nuclear Extract

Produkteigenschaften

• Konzentration: 1.0 mg/ml
• Puffer: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Quelle: Human
• Reinheit: The cells were grown in EMEM supplemented with 10% FBS (Fetal Bovine Serum). Cells were washed in PBS and incubated on ice in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris Cl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic ac
• Formulierung: Liquid (sterile filtered)

Anwendungsbeschreibung

• Application Verdünnung: WB: User Optimized
• Anwendungsbeschreibung: ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5