HeLa Cell Nuclear Extract TNFa Stimulated, Unconjugated

Produktübersicht

• Artikelname: HeLa Cell Nuclear Extract TNFa Stimulated, Unconjugated
• Artikelnummer: BYT-ORB348688
• Hersteller Artikelnummer: orb348688
• Alternativnummer: BYT-ORB348688-200
• Hersteller: Biorbyt
• Kategorie: Sonstiges
• Applikation: WB
• Konjugation: Unconjugated
• Alternative Synonym: HeLa Cell Nuclear Extract TNFa Stimulated, HeLa Nuclear Lysate TNF alpha Stimulated, HeLa TNF alpha Stimulated Nuclear Lysate, HeLa Lysate Tumor Necrosis Factor Nuclear Extract

Produktbeschreibung

HeLa Cell Nuclear Extract TNFa Stimulated

Produkteigenschaften

• Puffer: Preservative: None. Stabilizer: 10% (v/v) Glycerol. 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Quelle: Human
• Reinheit: The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). Cells were treated with 0.2 µg/ml TNFalpha for 30 min. The lysate was prepared by first washing the cells in PBS. Washed cells were then incubated on ice in lysis buffer containing
• Formulierung: Liquid (sterile filtered)

Anwendungsbeschreibung

• Application Verdünnung: WB: User Optimized
• Anwendungsbeschreibung: ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5