Lympholyte-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid

Produktübersicht

• Artikelname: Lympholyte-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid
• Artikelnummer: CED-CL5035
• Hersteller Artikelnummer: CL5035
• Alternativnummer: CED-CL5035
• Hersteller: Cedarlane
• Kategorie: Zellen/Zellkultur

Produktbeschreibung

Lympholyte-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid

Zusatzinformationen

Lympholyte(R)-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid

CEDARLANE’s Lympholyte® Cell Separation density gradient centrifugation media has been specifically designed for the isolation of viable PBMCs from mouse, rat, rabbit, human and other mammalian peripheral blood or tissues samples. The resulting cell population demonstrates a high, nonselective recovery of viable lymphocytes that are suitable for use as target cells in cytotoxicity and FACS assays and in in vivo / in vitro functional studies. All products are supplied as liquid with varying densities

Applications for Lympholyte® include:

• Isolation of viable lymphocytes from lymphoid organs by removal of red and dead cells.
• Removal of dead cells and debris:
  • From lymphocyte suspensions after treatement with antibody plus complement or following cell culture
  • Following sequential cytotoxicity studies eg. B-cell depletion.
  • From various clonal and hybridoma cell lines.
• Removal of erythrocytes, dead cells and debris from various clonal and hybridoma cell lines. (Lympholyte®-M and Lympholyte®-R)
• Removal of erythrocytes, dead cells and debris from other tissue suspensions including liver and lung. (Lympholyte®-M and Lympholyte®-R)
• Isolation of murine nuclear epidermal cells. (Lympholye®-M)
• Isolation of mononuclear cells from bone marrow preparations. (Lympholye®-H)

Lympholyte® Cell Separation Procedure

A. Gently layer the cell suspension or whole blood over top of the Lympholyte® making sure not to mix the two layers.

B. Alternatively, slowly add Lympholyte® to a Pasteur pipette allowing gravity to layer it under the whole blood or cell suspension.

C. Centrifuge the tube at room temperature for indicated time at the indicated speed.

D. Carefully remove the cells at the interface using a Pasteur pipette and transfer into a new centrifuge tube for washing.

Produkteigenschaften

• Lagerung: RT

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