Lympholyte-Rat Cell Separation Media (Isolation of lymphocytes from Rat lymphoid tissue), sterile liquid
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Artikelname: | Lympholyte-Rat Cell Separation Media (Isolation of lymphocytes from Rat lymphoid tissue), sterile liquid |
Artikelnummer: | CED-CL5041 |
Hersteller Artikelnummer: | CL5041 |
Alternativnummer: | CED-CL5041 |
Hersteller: | Cedarlane |
Kategorie: | Zellen/Zellkultur |
Lympholyte-Rat Cell Separation Media (Isolation of lymphocytes from Rat lymphoid tissue), sterile liquid |
Lympholyte(R)-Rat Cell Separation Media (Isolation of lymphocytes from Rat lymphoid tissue), sterile liquidCEDARLANE’s Lympholyte® Cell Separation density gradient centrifugation media has been specifically designed for the isolation of viable PBMCs from mouse, rat, rabbit, human and other mammalian peripheral blood or tissues samples. The resulting cell population demonstrates a high, nonselective recovery of viable lymphocytes that are suitable for use as target cells in cytotoxicity and FACS assays and in in vivo / in vitro functional studies. All products are supplied as liquid with varying densities Applications for Lympholyte® include:
Lympholyte® Cell Separation Procedure A. Gently layer the cell suspension or whole blood over top of the Lympholyte® making sure not to mix the two layers. B. Alternatively, slowly add Lympholyte® to a Pasteur pipette allowing gravity to layer it under the whole blood or cell suspension. C. Centrifuge the tube at room temperature for indicated time at the indicated speed. D. Carefully remove the cells at the interface using a Pasteur pipette and transfer into a new centrifuge tube for washing. |
Lagerung: | RT |