Anti-CD31 (Ms,Sw) from Rat (SZ31) for mouse FFPE tissue

Artikelnummer: DNA-DIA-310
Artikelname: Anti-CD31 (Ms,Sw) from Rat (SZ31) for mouse FFPE tissue
Artikelnummer: DNA-DIA-310
Hersteller Artikelnummer: DNA-DIA-310
Alternativnummer: DNA-DIA-310
Hersteller: dianova
Wirt: Rat
Kategorie: Antikörper
Applikation: IHC
Spezies Reaktivität: Mouse, Sw
Immunogen: Murines Aminosaure-Fragment (Aminosauren 610-681 von Maus CD31)
Konjugation: Unconjugated
Alternative Synonym: Platelet endothelial cell adhesion molecule,PECAM-1,EndoCAM,GPIIA,PECA1,CD31,PECAM1,Platelet/Endothelial Cell Adhesion Molecule 1,Platelet/Endothelial Cell Adhesion Molecule,PECAM1,Pecam,PECAM-1
Anti-CD31 (Ms,Sw) aus Ratte (SZ31) für Paraffingewebe aus Maus, IgG2a, Unconjugated, Monoclonal

Antibody clone SZ31 is the first antibody which reacts specifically with murine CD31 in formalin-fixed paraffin-embedded tissue sections.

 

Background

CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) is expressed constitutively on the surface of embryonic and adult endothelial cells. It is also expressed on cell surfaces of monocytes, neutrophils, platelets and certain T-cell subsets. It has been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is a 130kDa integral membrane glycoprotein and as a member of the immunoglobulin superfamily involved in the mediation of cell-to-cell adhesion. CD31-mediated endothelial cell-cell interactions play a major role in angiogenesis. Studies have shown CD31 to be a superior marker in human angiogenesis, which reportedly predicts tumor recurrence. Pathophysiological studies of CD31 in murine model systems had limitations because standard formalin-fixed sections were excluded. The clone SZ31 eliminates these restrictions by allowing high quality immunohistochemical (IHC) analysis of standard formalin-fixed paraffin sections in mice.

 

Instructions for Use

Immunohistochemical staining of standard formalin-fixed paraffin sections Indirect alkaline phosphatase staining (Other techniques, e.g. Avidin-Biotin-alkaline phosphatase (ABAP), alkaline phosphatase anti-alkaline phosphatase (APAAP) or horseradish peroxidase (HRP) -method are also possible).

1. Deparaffinize formalin-fixed paraffin-embedded mouse tissue sections by a standard procedure using xylol/ethanol

2. Antigen retrieval: high temperature heating of sections in citrate buffer pH 6,0 according to standard procedures

3. Block with 5% rabbit serum, 10 min RT

4. Wash with TBS, 3 x 5 min

5. Incubate with DIA-310 (1:10-1:20), 30min RT

6. Wash with TBS, 3 x 5 min

7. Incubate with rabbit anti-rat IgG (H+L) alkaline phosphatase (1:200), 30min RT

8. Wash with TBS, 3 x 5 min

9. Add substrate, e.g. Neufuchsin, 30min RT

10. Counterstain, e.g. with Hematoxylin-Papanicolaou

 

Product Specification

Artikelnummer DNA-DIA-310
Spezifität

CD31 (PECAM-1)

Spezies-Reaktivität

Maus, Schwein

Immunogen

Murines Aminosäure-Fragment (Aminosäuren 610-681 von Maus CD31)

Wirtsspezies

Ratte

Isotyp

IgG2a

Klon

SZ31

Klonalität (Mono-/Polyklonal)

monoklonal

Anwendung

Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe), Western Blot

Konjugation

unkonjugiert

Verdünnung

Immunhistochemie (IHC): 1:20, Western Blot (WB): 1:5.000

Format

0,05% NaN3, 2% BSA, gereinigter Antikörper (aus Kulturüberstand), in PBS (pH 7,4), Lyophilisat

Anwendungshinweis

Keine Kreuzreaktivität human CD31

Produktlinie / Thema

Angiogenese, CD-Marker

Zweckbestimmung

nur für Forschungszwecke

Temperatur - Lagerung

2-8°C

Temperatur - Transport

bei Raumtemperatur

Suchcode

DIA310

Hersteller / Marke

dianova

Uniprot_ID

Q08481

Gene_ID

18613

Alias

CD31, EndoCAM, GPIIA', PECA1, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, Platelet/Endothelial Cell Adhesion Molecule, Platelet/Endothelial Cell Adhesion Molecule 1

Klonalität: Monoclonal
Konzentration: (0,2mg/ml)
Klon-Bezeichnung: SZ31
Isotyp: IgG2a
NCBI: 18613
UniProt: Q08481
Puffer: in PBS, 2% BSA, 0,05%NaN3
Lagerung: 2-8 °C
Target-Kategorie: CD31
Application Verdünnung: Immunhistochemie (IHC): 1:20,Western Blot (WB): 1:5.000
Mouse Aorta (FFPE): anti-CD31 staining with clone SZ31
Mouse Endocardium (FFPE): anti-CD31 staining with clone SZ31
Murine adenocarcinoma. anti-CD31 staining with clone SZ31
Murine Spinal Cord: anti-CD31 staining with clone SZ31
Western Blot of murine cell lysate with antibody clone SZ31
Aortic-origin CD31 staining with clone SZ31
Bone-Marrow (IHC-P) - Staining with anti-CD31 Antibody clone SZ31
Mouse Brain (IHC-P) - Staining with anti-CD31 Antibody
murine Colon (IHC-P) - Staining with anti-CD31 Antibody (SZ31)
murine Kidney (IHC-P) - Staining with anti-CD31 Antibody (SZ31)
murine Liver (IHC-P) - Staining with anti-CD31 Antibody (SZ31)
Mouse Lung CD31 Staining Antibody SZ31
Mouse Lymph-nodes
Mosue Mesenteric vessels
Murine adenocarcinoma. anti-CD31 staining with clone SZ31 (higher magnification)
Mouse Skeletal muscle
Mouse Pancreas (FFPE)
CD31 in Small intestine (Ms) - Staining with anti-CD31)
CD31 in Small intestine (Ms) (higher magnification) (Staining with anti-CD31)