The mouse monoclonal anti-(His)6-tag antibody, clone 13/45/31-2 (H. Zentgraf/DKFZ Heidelberg, Germany), specifically detects any kind of histidine-tagged proteins in cells and complex cellular lysates. This monoclonal antibody specifically reacts with recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or internally. A higher number of histidine residues leads to an increased binding affinity of the antibody, e.g. an expression construct with 10 histidine residues increases the affinity about 10- to 20-fold. Additional flanking amino acids are not required for antibody binding, therefore enabling the choice of many different expression vectors on the only condition that the TAG-epitope is sterically available.
The Antibody clone 13/45/31-2 /13/45/31 is with more than 100 citations one of the most cited His-Tag antibodies world wide.
Background
Recombinant proteins are utilized for various purposes in molecular biology. Many prokaryotic expression vectors have been established enabling synthesis of the protein of interest as a fusion with a peptide, thus facilitating purification. Expression of recombinant proteins in E. coli as a fusion protein that includes histidine residues (His) as tag is one of the most popular methods, because histidine tagged proteins have useful attributes. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbant and the resulting protein can be selectively detected using antibodies such as clone 13/45/31-2.
Product Specifications
| Artikelnummer |
DIA-900-100 |
| Spezifität |
6-His Epitop-Tag, His Epitop-Tag, His-tag (N/C-terminal)
|
| Spezies-Reaktivität |
alle, rekombinant
|
| Immunogen |
Rekombinantes (HIS)6-p53-Protein
|
| Wirtsspezies |
Maus
|
| Isotyp |
IgG1
|
| Klon |
13/45/31
|
| Klonalität (Mono-/Polyklonal) |
monoklonal
|
| Anwendung |
Durchflusszytometrie (Flow Cytometry), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Western Blot
|
| Konjugation |
unkonjugiert
|
| Verdünnung |
Durchflusszytometrie 5 – 10 µl/10^6 Zellen, ELISA 0,1 – 0,4 µg/ml, Immunfluoreszenz 1 – 4 µg/ml, Immunhistochemie (IHC): 1 – 4 µg/ml, Immunpräzipitation 4 µg/ml, Western Blot (WB): 1 – 4 µg/ml
|
| Format |
0,05% NaN3, 6% BSA, in PBS (pH 7,4), ProteinA/G-gereinigter Antikörper (aus Kulturüberstand)
|
| Anwendungshinweis |
Achtung geänderte Konzentration: Jetzt 1 mg/ml!
|
| Produktlinie / Thema |
Epitop-Tags, Haptene und Label
|
| Zweckbestimmung |
nur für Forschungszwecke
|
| Temperatur - Lagerung |
2-8°C
|
| Temperatur - Transport |
bei Raumtemperatur
|
| Suchcode |
6his, anti his, DIA900, hexahistidine, his6, histag, histidin tag, histidine, polyhistidine
|
| Hersteller / Marke |
dianova
|
| Uniprot_ID |
N/A
|
| Gene_ID |
N/A
|
| Bestellhinweis |
Neue Produktnummer, ersetzt dia-900 (Neue Konzentration!)
|
| Alias |
6*His, 6His, 6histag, 6XHis, hexahistidine, HHHHHH, His Epitop-Tag, His Epitope-Tag, His Tag, his6, histidin tag, polyhistidine
|
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