p53 antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX50438
Artikelname: p53 antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX50438
Hersteller Artikelnummer: GTX50438
Alternativnummer: GTX50438-100
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC-P, WB, IF, ICC, IP
Spezies Reaktivität: Rat, Human, Mouse
Immunogen: Peptide sequence around aa.13~17 (P-L-S-Q-E) derived from human p53.
Konjugation: Unconjugated
Alternative Synonym: tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Klonalität: Polyclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 44
Isotyp: IgG
NCBI: 7157
UniProt: P04637
Puffer: PBS (without Mg?? and Ca??) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: tumor protein p53
Application Verdünnung: WB: 1:500-1:1000. ICC/IF: 1:100-1:200. IHC-P: 1:50-1:100. IP: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:500-1:1000. ICC/IF: 1:100-1:200. IHC-P: 1:50-1:100. IP: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
IHC-P analysis of rat colon tissue sections using GTX50438 p53 antibody.
IHC-P analysis of rat breast tissue sections using GTX50438 p53 antibody.
IHC-P analysis of rat breast tissue using GTX50438 p53 antibody.
Dilution : 1:100
WB analysis of extracts from mouse brain tissue using GTX50438 p53 antibody.
IP analysis of extracts from 293T cells using GTX50438 p53 antibody.
ICC/IF analysis of methanol-fixed HeLa cells using GTX50438 p53 antibody.
WB analysis of extracts from various sample using GTX50438 p53 antibody.
Lane 1 : rat breast tissue
Lane 2 : rat colon tissue
Dilution : 1:1000
IHC-P analysis of human breast carcinoma tissue using GTX50438 p53 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX50438) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.