Histone H3K9me1 (mono-methyl Lys9) antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX54101
Artikelname: Histone H3K9me1 (mono-methyl Lys9) antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX54101
Hersteller Artikelnummer: GTX54101
Alternativnummer: GTX54101-100
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC-P, IF, ICC, DOT, ChIP assay, WB
Spezies Reaktivität: Rat, Human, Mouse
Immunogen: A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
Konjugation: Unconjugated
Alternative Synonym: histone cluster 3 H3 , H3.4 , H3/g , H3FT , H3t
Klonalität: Polyclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 16
Isotyp: IgG
NCBI: 8290
UniProt: Q16695
Puffer: PBS pH7.3, 0.02% sodium azide, 50% glycerol.
Reinheit: Purified by affinity chromatography
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: histone cluster 3 H3
Application Verdünnung: WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. Dot: Assay dependent. ChIP assay: 1:20-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:50-1:200. Dot: Assay dependent. ChIP assay: 1:20-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
ChIP analysis of 293 cells using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
ICC/IF analysis of NIH/3T3 cells using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Blue : DAPI
Dilution : 1:100
IHC-P analysis of rat testis tissue using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Dilution : 1:200
ICC/IF analysis of C6 cells using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Blue : DAPI
Dilution : 1:100
Dot blot analysis of various methylation peptides using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
IHC-P analysis of human embryo brain tissue using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Dilution : 1:200
ICC/IF analysis of HeLa cells using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Blue : DAPI
Dilution : 1:100
ICC/IF analysis of 293T cells using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Blue : DAPI
WB analysis of various sample lysates using GTX54101 Histone H3K9me1 (mono-methyl Lys9) antibody.
Loading : 25ug per lane