Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX60337
Artikelname: Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX60337
Hersteller Artikelnummer: GTX60337
Alternativnummer: GTX60337-50
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, ICC, DOT, ChIP assay, WB, ELISA
Spezies Reaktivität: Human, Mouse
Immunogen: The region of histone H4 containing the acetylated lysines 5, 8 and 12 (H4K5,8,12ac), using a KLH-conjugated synthetic peptide.
Konjugation: Unconjugated
Alternative Synonym: H4 histone family, member A, H4FA, histone 1, H4a, histone cluster 1, H4a, histone H4
Klonalität: Polyclonal
Konzentration: 0.76 mg/ml (Please refer to the vial label for the specific concentration.)
Isotyp: IgG
NCBI: 8359
Puffer: PBS, 0.05% sodium azide, 0.05% ProClin 300
Reinheit: Purified by affinity chromatography
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: histone cluster 1, H4a
Application Verdünnung: WB: 1:1,000. ICC/IF: 1:500. Dot: 1:20,000. ELISA: 1:1,000. ChIP assay: 0.5-5 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: ChIP/ChIP-seq (0.5-1 µg/IP), ELISA (1:1000), Dot blotting (1:20,000), Western blotting (1:1,000), Immunofluorescence (1:500)
ICC/IF analysis of HeLa cells using H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with the H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
ICC/IF analysis of HeLa cells using H4K5acK8acK12ac peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H4K5acK8acK12ac peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
ELISA was performed using a serial dilution of Histone H4K5acK8acK12ac (acetyl Lys5, Lys8, Lys12) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,500.
ICC/IF analysis of HeLa cells using H4K5K8K12 unmodified peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H4K5K8K12 unmodified peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
ICC/IF analysis of HeLa cells using H4K5acK8acK12acK16ac peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H4K5acK8acK12acK16ac peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
ICC/IF analysis of HeLa cells using H2A.ZK5acK7acK11ac peptite-blocked H4K5acK8acK12ac antibody and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Cells were immunofluorescently labeled with H2A.ZK5acK7acK11ac peptite-blocked H4K5acK8acK12ac antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H4K5acK8acK12ac antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
Dot blot analysis of peptides containing other histone modifications and the unmodified H4 using H4K5acK8acK12ac antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
ChIP was performed with NB4 nuclear extract and either 1 μg of H4K5,8,12ac antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to 1 kb upstream of the GAPDH gene or Sat2.