NUP98 antibody [2H10], IgG2c, Unconjugated, Rat, Monoclonal

Artikelnummer: GTX00697
Artikelname: NUP98 antibody [2H10], IgG2c, Unconjugated, Rat, Monoclonal
Artikelnummer: GTX00697
Hersteller Artikelnummer: GTX00697
Alternativnummer: GTX00697-100
Hersteller: GeneTex
Wirt: Rat
Kategorie: Antikörper
Applikation: ELISA, IF, ICC, WB, DOT
Spezies Reaktivität: Yeast, Rat, Human, Mouse, Schizosaccharomyces pombe
Immunogen: Recombinant GST-fused human Nup98 (amino acids 1-466)
Konjugation: Unconjugated
Alternative Synonym: nucleoporin 98 , ADIR2 , NUP196 , NUP96
Klonalität: Monoclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: 2H10
Molekulargewicht: 198
Isotyp: IgG2c
NCBI: 4928
UniProt: P52948
Puffer: Filter-sterilized PBS, 50% glycerol
Reinheit: Purified IgG
ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10].
The dots are Nup98.
WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10].
Dilution : 1 μg/ml
WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10].
Dilution : 1:2000
ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10].
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.
WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10].
Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe.
Dilution : 2 μg/ml