SOD1 antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SOD1 antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX100554
• Hersteller Artikelnummer: GTX100554
• Alternativnummer: GTX100554-100,GTX100554-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IHC-P, WB
• Spezies Reaktivität: Aves, Human, Mouse, Rat, Reptile
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SOD1. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.15 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 16
• NCBI: 6647
• UniProt: P00441
• Puffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX100554 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX100554 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SOD1 antibody (GTX100554) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SOD1 antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence photomicrographs of paraffin-embedded mouse fetal brain.
Green: SOD1 antibody (GTX100554) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (50 μg of whole cell lysate)
A: Rat brain
15% SDS PAGE
GTX100554 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SOD1 antibody detects SOD1 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOD1 protein stained by SOD1 antibody (GTX100554) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.

ELISA detection of SOD1 using GTX100554 for capture at a concentration of 5 μg/mL and GTX89049 for detection at a concentration of 1.5 μg/mL.