Heme Oxygenase 1 antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX101147
Artikelname: Heme Oxygenase 1 antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX101147
Hersteller Artikelnummer: GTX101147
Alternativnummer: GTX101147-100,GTX101147-25
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, ICC, IHC-P, WB
Spezies Reaktivität: Human, Monkey, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: heme oxygenase 1 , HMOX1D , HO-1 , HSP32 , bK286B10
Klonalität: Polyclonal
Konzentration: 0.45 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 33
NCBI: 3162
UniProt: P09601
Puffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX101147 ELISA Image
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human renal carcinoma .
Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min