LOXL2 antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: LOXL2 antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX105085
• Hersteller Artikelnummer: GTX105085
• Alternativnummer: GTX105085-100,GTX105085-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IHC-P, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: lysyl oxidase like 2 , LOR , LOR2 , WS9-14

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.86 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 87
• Sensitivitaet: Mouse reactivity has been validated by IHC-P only, not yet been tested by other applications.
• NCBI: 4017
• UniProt: Q9Y4K0
• Puffer: 1XPBS, 20% Glycerol (pH7), 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX105085 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LOXL2 antibody detects LOXL2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:3000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: LOXL2 protein stained by LOXL2 antibody (GTX105085) diluted at 1:500..
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LOXL2 antibody detects LOXL2 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min