Rad21 antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Rad21 antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX106012
• Hersteller Artikelnummer: GTX106012
• Alternativnummer: GTX106012-100,GTX106012-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, ICC, IHC-P, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Rad21. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: RAD21 cohesin complex component , CDLS4 , HR21 , HRAD21 , MCD1 , MGS , NXP1 , SCC1 , hHR21

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.34 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 72
• NCBI: 5885
• UniProt: O60216
• Puffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX106012 IHC-P Image

Rad21 antibody detects Rad21 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using RAD21(GTX106012) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX106012 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of RAD21 protein from Jurkat whole cell extracts using 5 μg of RAD21 antibody (GTX106012).
Western blot analysis was performed using RAD21 antibody (GTX106012).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (GTX106012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

RAD21 antibody detects RAD21 protein at nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
RAD21 antibody (GTX106012) diluted at 1:250.

Rad21 antibody detects Rad21 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rad21 antibody detects Rad21 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min