Rad21 antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit

Artikelnummer: GTX106012
Artikelname: Rad21 antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit
Artikelnummer: GTX106012
Hersteller Artikelnummer: GTX106012
Alternativnummer: GTX106012-100,GTX106012-25
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, WB, IHC-P, IP
Spezies Reaktivität: Rat, Mouse, Human
Immunogen: Recombinant protein encompassing a sequence within the center region of human Rad21. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: RAD21 cohesin complex component , CDLS4 , HR21 , HRAD21 , MCD1 , MGS , NXP1 , SCC1 , hHR21
Klonalität: Polyclonal
Konzentration: 0.44 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 72
Isotyp: IgG
NCBI: 5885
UniProt: O60216
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RAD21(GTX106012) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Rad21 antibody detects Rad21 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
RAD21 antibody detects RAD21 protein at nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary adenocarcinoma.
RAD21 antibody (GTX106012) diluted at 1:250.
Immunoprecipitation of RAD21 protein from Jurkat whole cell extracts using 5 μg of RAD21 antibody (GTX106012).
Western blot analysis was performed using RAD21 antibody (GTX106012).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad21 antibody (GTX106012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using RAD21(GTX106012) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX106012 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Rad21 antibody detects Rad21 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rad21 antibody detects Rad21 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Rad21 stained by Rad21 antibody (GTX106012) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min