PGK1 antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit

Artikelnummer: GTX107614
Artikelname: PGK1 antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit
Artikelnummer: GTX107614
Hersteller Artikelnummer: GTX107614
Alternativnummer: GTX107614-100,GTX107614-25
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IP, PLA, ELISA, ICC, IHC-P, WB
Spezies Reaktivität: Rat, Yeast, Mouse, Human
Immunogen: Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA
Klonalität: Polyclonal
Konzentration: 0.048 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 45
Isotyp: IgG
NCBI: 5230
UniProt: P00558
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
PGK1 antibody detects PGK1 protein by western blot analysis.
A. 50 μg Rat liver lysate/extract
10% SDS-PAGE
PGK1 antibody (GTX107614) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of methanol-fixed HeLa, using PGK1(GTX107614) antibody at 1:50 dilution.
PGK1 antibody detects PGK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGK1 protein stained by PGK1 antibody (GTX107614) diluted at 1:20.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
PGK1 antibody detects PGK1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PGK1 antibody detects PGK1 protein in 1% O2-treated samples by western blot analysis.
Upper panel: PGK1 antibody (GTX107614)
Lower panel: beta Actin antibody (GTX109639)
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 24hr)
10% SDS PAGE?
PGK1 antibody (GTX107614) dilution: 1:1000
beta Actin antibody (GTX109639) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX107614 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded human hepatoma, using PGK1 antibody(GTX107614) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.