SLC25A13 antibody [N3C2], Internal, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SLC25A13 antibody [N3C2], Internal, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX109001
• Hersteller Artikelnummer: GTX109001
• Alternativnummer: GTX109001-100,GTX109001-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SLC25A13. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: solute carrier family 25 member 13 , ARALAR2 , CITRIN , CTLN2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.61 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 74
• NCBI: 10165
• UniProt: Q9UJS0
• Puffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX109001 WB Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using SLC25A13(GTX109001) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX109001 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:1000 and competitor's antibody (10789-1-AP) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLC25A13 protein stained by SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500.
Blue: Hoechst 33342 staining.

SLC25A13 antibody [N3C2], Internal detects SLC25A13 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wild-type (WT) and SLC25A13 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC25A13 antibody [N3C2], Internal (GTX109001) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.