EEA1 antibody [C3], C-term, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: EEA1 antibody [C3], C-term, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX109638
• Hersteller Artikelnummer: GTX109638
• Alternativnummer: GTX109638-100,GTX109638-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, WB
• Spezies Reaktivität: Hamster, Human, Mouse, Rat
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: early endosome antigen 1 , MST105 , MSTP105 , ZFYVE2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 162
• NCBI: 8411
• UniProt: Q15075
• Puffer: 1XPBS (pH7), 20% Glycerol, 0.01% Thimerosal.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX109638 WB Image

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.
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Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EEA1(GTX109638) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg A375 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX109638 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

EEA1 antibody [C3], C-term detects EEA1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EEA1 protein stained by EEA1 antibody [C3], C-term (GTX109638) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).