EEA1 antibody [C3], C-term, IgG, Clone: Polyclonal, Unconjugated, Rabbit

Artikelnummer: GTX109638
Artikelname: EEA1 antibody [C3], C-term, IgG, Clone: Polyclonal, Unconjugated, Rabbit
Artikelnummer: GTX109638
Hersteller Artikelnummer: GTX109638
Alternativnummer: GTX109638-100,GTX109638-25
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, WB, IHC-P
Spezies Reaktivität: Rat, Mouse, Hamster, Human
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: early endosome antigen 1 , MST105 , MSTP105 , ZFYVE2
Klonalität: Polyclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 162
Isotyp: IgG
NCBI: 8411
UniProt: Q15075
Puffer: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.
_x000D_
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
EEA1 antibody [C3], C-term detects EEA1 protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg A375 whole cell lysate/extract
5% SDS-PAGE
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX109638 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
EEA1 antibody [C3], C-term detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
EEA1 antibody [C3], C-term detects EEA1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EEA1 protein stained by EEA1 antibody [C3], C-term (GTX109638) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EEA1(GTX109638) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with EEA1 antibody [C3], C-term (GTX109638) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.