Hsp90 alpha antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit

Artikelnummer: GTX109753
Artikelname: Hsp90 alpha antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit
Artikelnummer: GTX109753
Hersteller Artikelnummer: GTX109753
Alternativnummer: GTX109753-100,GTX109753-25
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, WB, IHC-P, IP
Spezies Reaktivität: Mouse, Human, Rat, Other, Monkey
Immunogen: Recombinant protein encompassing a sequence within the center region of human Hsp90 alpha. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: heat shock protein 90 alpha family class A member 1 , EL52 , HEL-S-65p , HSP86 , HSP89A , HSP90A , HSP90N , HSPC1 , HSPCA , HSPCAL1 , HSPCAL4 , HSPN , Hsp103 , Hsp89 , Hsp90 , LAP-2 , LAP2
Klonalität: Polyclonal
Konzentration: 0.27 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 85
Isotyp: IgG
NCBI: 3320
UniProt: P07900
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Hsp90 alpha antibody (GTX109753) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 μg of Hsp90 alpha antibody (GTX109753).
Western blot analysis was performed using Hsp90 alpha antibody (GTX109753).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:500.
Blue: Hoechst 33342 staining.
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min