COL1A1 antibody [N1N2], N-term, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX112731
Artikelname: COL1A1 antibody [N1N2], N-term, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX112731
Hersteller Artikelnummer: GTX112731
Alternativnummer: GTX112731-100,GTX112731-25
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IHC-Fr, IHC-P, IP, WB
Spezies Reaktivität: Fish, Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human COL1A1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4
Klonalität: Polyclonal
Konzentration: 1.29 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 139
NCBI: 1277
UniProt: P02452
Puffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX112731 IHC-P Image
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation of COL1A1 protein from SK-N-AS whole cell extracts using 5 μg of COL1A1 antibody [N1N2], N-term (GTX112731).
Western blot analysis was performed using COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
COL1A1 antibody detects COL1A1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody (GTX112731) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.