PABP antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PABP antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX113954
• Hersteller Artikelnummer: GTX113954
• Alternativnummer: GTX113954-100,GTX113954-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PABP. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: poly(A) binding protein cytoplasmic 1 , PAB1 , PABP , PABP1 , PABPC2 , PABPL1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 71
• NCBI: 26986
• UniProt: P11940
• Puffer: 1XPBS (pH7), 20% Glycerol, 0.01% Thimerosal.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX113954 WB Image

PABP antibody detects PABP protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PABP antibody (GTX113954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PABP antibody detects PABP protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PABP antibody (GTX113954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PABP antibody detects PABP protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PABP antibody (GTX113954) dilution: 1:500.
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Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

PABP antibody detects PABP protein at cytoplasm by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PABP protein stained by PABP antibody (GTX113954) diluted at 1:500.
Blue: Hoechst 33342 staining.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody (GTX113954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PABP(GTX113954) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PABP antibody (GTX113954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.