c-Kit (phospho Tyr823) antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: c-Kit (phospho Tyr823) antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX25634
• Hersteller Artikelnummer: GTX25634
• Alternativnummer: GTX25634-50
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, WB
• Spezies Reaktivität: Aves, Human, Mouse
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 823. The sequence is conserved in cow and dog.
• Konjugation: Unconjugated
• Alternative Synonym: KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR

Produktbeschreibung

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known a

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: Batch dependent (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: Polyclonal
• Molekulargewicht: 110
• Isotyp: IgG
• NCBI: 3815
• UniProt: P10721
• Puffer: PBS pH7.3, 1mg/ml BSA, 50% Glycerol, 0.05% Sodium azide.
• Quelle: Human
• Reinheit: Purified by antigen-affinity chromatography
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: ICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX25634 WB Image

ICC/IF analysis of Jurkat cells treated with 250 ng/ml SCF for 5 minutes using GTX25634 c-Kit (phospho Tyr823) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes