c-Kit (phospho Tyr936) antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX25635
Artikelname: c-Kit (phospho Tyr936) antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX25635
Hersteller Artikelnummer: GTX25635
Alternativnummer: GTX25635-50
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Kits/Assays
Applikation: IF, ICC, WB, FACS
Spezies Reaktivität: Human
Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 936. The sequence is conserved in cow.
Konjugation: Unconjugated
Alternative Synonym: KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR
Klonalität: Polyclonal
Konzentration: Batch dependent (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 110
Isotyp: IgG
NCBI: 3815
UniProt: P10721
Puffer: PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide
Reinheit: Purified by antigen-affinity chromatography
ICC/IF analysis of A549 cells using GTX25635 c-Kit (phospho Tyr936) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature
WB analysis of M07e cells left untreated (Lane 1) or treated (Lane 2-5) with SCF using GTX25635 c-Kit (phospho Tyr936) antibody.
WB analysis of whole cell extracts (30 μg lysate) of K562 (Lane 1) treated for 10 minutes with 100 ng/ml of SCF (Lane 2), HT 29 (Lane 3) treated for 10 minutes with 100 ng/ml of SCF (Lane 4) using GTX25635 c-Kit (phospho Tyr936) antibody.
Dilution : 1:250