HIF2 alpha antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit

Artikelnummer: GTX30114
Artikelname: HIF2 alpha antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit
Artikelnummer: GTX30114
Hersteller Artikelnummer: GTX30114
Alternativnummer: GTX30114-100
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IHC, WB, IHC-P
Spezies Reaktivität: Mouse, Human, Rat, Monkey, Fish
Immunogen: A peptide derived from the mouse/human HIF2 alpha.
Konjugation: Unconjugated
Alternative Synonym: endothelial PAS domain protein 1 , HIF-2alpha , HIF2A , HLF , HRF , MOP2 , bHLHe73
Klonalität: Polyclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 97
Isotyp: IgG
NCBI: 13819
UniProt: P97481
Puffer: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Immunocytochemistry/Immunofluorescence: HIF-2 alpha Antibody (GTX30114) - Detection of HIF-2 Alpha (Green) in RCC4 cells using GTX30114. Nuclei (Blue) are counterstained with Hoechst 33258.
Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using GTX30114
HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.
Blue: Hoechst 33342 staining.
Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis on normoxic and hypoxic nuclear rat cell lysates.
Immunohistochemistry: HIF-2 alpha Antibody (GTX30114) - Hif-2 alpha immunoreactivity in human cardiac myocytes
HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.
Blue: Hoechst 33342 staining.
GTX30114 utilized in western blot with PC12 nuclear extracts, 1:1,000, 5 second exposure.
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 0.01 O2 treatment for 24h) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Non-transfected (-) and HIF2A-transfected (+, including Myc-DDK-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.