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GTX30114 ICC/IF Image |
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Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using GTX30114 |
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HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.Blue: Hoechst 33342 staining. |
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Western Blot: HIF-2 alpha Antibody (GTX30114) - Analysis on normoxic and hypoxic nuclear rat cell lysates. |
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Immunohistochemistry: HIF-2 alpha Antibody (GTX30114) - Hif-2 alpha immunoreactivity in human cardiac myocytes |
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HIF2 alpha antibody detects HIF2 alpha protein at nucleus by immunofluorescent analysis.Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIF2 alpha stained by HIF2 alpha antibody (GTX30114) diluted at 1:200.Blue: Hoechst 33342 staining. |
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GTX30114 utilized in western blot with PC12 nuclear extracts, 1:1,000, 5 second exposure. |
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HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 0.01 O2 treatment for 24h) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Non-transfected (-) and HIF2A-transfected (+, including Myc-DDK-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX30114) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |