p53 antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: p53 antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX50438
• Hersteller Artikelnummer: GTX50438
• Alternativnummer: GTX50438-100
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Peptide sequence around aa.13~17 (P-L-S-Q-E) derived from human p53.
• Konjugation: Unconjugated
• Alternative Synonym: tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Produktbeschreibung

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle ar

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: Polyclonal
• Molekulargewicht: 44
• Isotyp: IgG
• NCBI: 7157
• Pubmed: 32890980,
• UniProt: P04637
• Puffer: PBS (without Mg and Ca) pH7.4, 150mM NaCl, 50% Glycerol, 0.02% Sodium azide.
• Quelle: Human
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:1000. ICC/IF: 1:100-1:200. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

IP analysis of extracts from 293T cells using GTX50438 p53 antibody.

WB analysis of extracts from mouse brain tissue using GTX50438 p53 antibody.

IHC-P analysis of rat breast tissue sections using GTX50438 p53 antibody.

IHC-P analysis of rat colon tissue sections using GTX50438 p53 antibody.

IHC-P analysis of rat breast tissue using GTX50438 p53 antibody.
Dilution : 1:100

ICC/IF analysis of methanol-fixed HeLa cells using GTX50438 p53 antibody.

GTX50438 WB Image

IHC-P analysis of human breast carcinoma tissue using GTX50438 p53 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX50438) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.