SARS-CoV-2 (COVID-19) Nucleocapsid overexpression 293T whole cell lysate

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) Nucleocapsid overexpression 293T whole cell lysate
• Artikelnummer: GTX535665
• Hersteller Artikelnummer: GTX535665
• Alternativnummer: GTX535665-200
• Hersteller: GeneTex
• Kategorie: Proteine/Peptide
• Applikation: ELISA, WB
• Spezies Reaktivität: Virus
• Alternative Synonym: 2019-nCoV NP, 2019-nCoV Nucleocapsid, COVID-19 NP, COVID-19 Nucleocapsid, SARS-CoV-2 NP, SARS-CoV-2 Nucleocapsid, covid-19, sars-cov-2

Produktbeschreibung

Whole-cell lysate of 293T cells overexpressing SARS-CoV-2 nucleocapsid protein (full-length, N-terminal 3x Flag-tagged). The translated amino acid sequence is based on QHD43423.2. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Pl

Produkteigenschaften

• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Puffer: 50 mM Tris-HCl (pH8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 µg/ml Leupeptin, 2 µg/ml Aprotinin, 1 µg/ml Pepstatin A, 0.025% ProClin 300.
• Formulierung: Liquid

Bilder

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-transfected and transfected 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX535665 WB Image