SARS-CoV-2 (COVID-19) nucleocapsid overexpression 293T whole cell lysate

Artikelnummer: GTX535665
Artikelname: SARS-CoV-2 (COVID-19) nucleocapsid overexpression 293T whole cell lysate
Artikelnummer: GTX535665
Hersteller Artikelnummer: GTX535665
Alternativnummer: GTX535665-200
Hersteller: GeneTex
Kategorie: Zellen/Zellkultur
Applikation: ELISA, WB
Spezies Reaktivität: SARS Coronavirus 2
Alternative Synonym: SARS-CoV-2 Nucleocapsid, COV2, COVID, SARS-COV, SARS-CoV-2 NP, CORONA, COVID19, COVID-19, COVID-19 Nucleocapsid, COVID-19 NP, COV-2, NCOV, 2019-nCoV NP, SARS-COV-2, 2019-nCoV Nucleocapsid
Konzentration: 1 mg/ml
Puffer: 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 µg/ml Leupeptin, 2 µg/ml Aprotinin, 1 µg/ml Pepstatin A, 0.025% ProClin 300
Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Non-transfected and transfected 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SARS-CoV-2 (COVID-19) nucleocapsid 293T overexpression cell lysates (GTX535665) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.