SARS-CoV Spike overexpression 293T whole cell lysate

Artikelnummer: GTX535668
Artikelname: SARS-CoV Spike overexpression 293T whole cell lysate
Artikelnummer: GTX535668
Hersteller Artikelnummer: GTX535668
Alternativnummer: GTX535668-200
Hersteller: GeneTex
Kategorie: Proteine/Peptide
Applikation: WB
Spezies Reaktivität: SARS Coronavirus
Alternative Synonym: NCOV, COV2, CORONA, COVID19, SARS-COV-2, SARS-COV, COV-2, COVID, COVID-19
Konzentration: 1 mg/ml
Puffer: 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 µg/ml Leupeptin, 2 µg/ml Aprotinin, 1 µg/ml Pepstatin A, 0.025% ProClin 300
SARS-CoV spike 293T overexpression cell lysates (GTX535668) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV Spike (SΔ3) antibody [7G12] (GTX632603) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV Spike (SΔ3) antibody [7G12] (GTX632603) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.