Protocol 1: Conjugation of Janelia Fluor 549 SE to antibodies Using a desalting column, perform buffer exchange (following manufacturer instructions) of antibody into a carbonate buffer (100mM, pH 8-8.25) Mix together the antibody and Janelia Fluor 54
β-tubulin expression in HeLa cells visualised using a Janelia Fluor® 549 conjugated secondary antiobody.
GFAP expression in rat brain visualised using a Janelia Fluor® 549 conjugated secondary antibody.
β-tubulin expression in HeLa cells visualised using a Janelia Fluor® 549 conjugated secondary antiobody.
Fig1. Excitation and emission spectra of Janelia Fluor® 549, SE.
HB7336
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