Streptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.
Western Blot:- 0.5-2 µg / ml Histo-/Cyto-Chemistry:- 0.5-2 µg / ml
Pax2 expression in astrocytes of aged rat retina
Tissue specimen of 19 month old (aged) Wistar rat retina illustrates Pax2 expression in retinal astrocytes in the central retina. Triple label immunohistochemistry was executed on retinal wholemount preparations. Pax2 stained for surrounding nuclei of astrocytes (red, biotinylated anti-rabbit Ig, Cy3-conjugated streptavidin - Jackson ImmunoResearch 016-160-084), DAPI stain for cell nuclei (blue), and GFAP stained for astrocytes (green, Alexa 488-conjugated anti-mouse IgG1).
Mansour, H., Chamberlain, C. G., Weible II, M. W., Hughes, S., Chu, Y. and Chan-Ling, T. (2008), Aging-related changes in astrocytes in the rat retina: imbalance between cell proliferation and cell death reduces astrocyte availability. Aging Cell, 7: 526–540. doi: 10.1111/j.1474-9726.2008.00402.x
Confocal micrograph of the ventral horn in the D9 spinal cord segment of a red-eared turtle (Trachemys scripta elegans). The composite image of an electrophysiologically measured and biocytin-injected motor neuron (red, Cy3 016-160-084), in a population of motor neurons (green, Choline acetyltransferase, Alexa Fluor® 488 705-545-147) with a nuclear staining (blue, DAPI).
Kristian Reveles Jensen, University of Copenhagen.
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