Alexa Fluor 488-conjugated AffiniPure(TM) F(ab)2 Fragment Goat Anti-Human IgM, Fc5µ Fragment Specific (min X Bov Sr Prot)
Artikelnummer:
JIM-109-546-129
Hersteller Artikelnummer:
109-546-129
Alternativnummer:
JIM-109-546-129
Hersteller:
Jackson ImmunoResearch
Wirt:
Goat
Kategorie:
Antikörper
Spezies Reaktivität:
Human
Konjugation:
Alexa Fluor® 488
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with IgM from other species.
F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Klonalität:
Polyclonal
Isotyp:
F(ab')₂ Fragment
Puffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Reinheit:
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Formulierung:
F(ab')₂ Fragment
Antibody Type:
Secondary Antibody
Application Verdünnung:
1:100 - 1:800 for most applications
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
