Peroxidase-conjugated AffiniPure(TM) F(ab)2 Fragment Goat Anti-Rabbit IgG, Fc Fragment Specific (min X Hu Sr Prot)
Artikelnummer:
JIM-111-036-046
Hersteller Artikelnummer:
111-036-046
Alternativnummer:
JIM-111-036-046
Hersteller:
Jackson ImmunoResearch
Wirt:
Goat
Kategorie:
Antikörper
Spezies Reaktivität:
Rabbit
Konjugation:
Horseradish Peroxidase
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of rabbit IgG heavy chain but not with the Fab portion of rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.
F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Klonalität:
Polyclonal
Isotyp:
F(ab')₂ Fragment
Puffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Reinheit:
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Formulierung:
F(ab')₂ Fragment
Antibody Type:
Secondary Antibody
Application Verdünnung:
1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
