Biotin-SP-conjugated AffiniPure(TM) Fab Fragment Goat Anti-Rat IgG (H+L)

Produktübersicht

• Artikelname: Biotin-SP-conjugated AffiniPure(TM) Fab Fragment Goat Anti-Rat IgG (H+L)
• Artikelnummer: JIM-112-067-003
• Hersteller Artikelnummer: 112-067-003
• Alternativnummer: JIM-112-067-003
• Hersteller: Jackson ImmunoResearch
• Wirt: Goat
• Kategorie: Antikörper
• Spezies Reaktivität: Rat
• Konjugation: Biotin-SP (long spacer)

Zusatzinformationen

Based on antigen-binding assay and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

 

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Produkteigenschaften

• Klonalität: Polyclonal
• Isotyp: Fab Fragment
• Puffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
• Reinheit: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
• Formulierung: Fab Fragment
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: 1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry