Biotin-SP-conjugated AffiniPure(TM) Rabbit Anti-Rat IgG + IgM (H+L) (min X Hu Sr Prot)

Artikelnummer: JIM-312-065-048
Artikelname: Biotin-SP-conjugated AffiniPure(TM) Rabbit Anti-Rat IgG + IgM (H+L) (min X Hu Sr Prot)
Artikelnummer: JIM-312-065-048
Hersteller Artikelnummer: 312-065-048
Alternativnummer: JIM-312-065-048
Hersteller: Jackson ImmunoResearch
Wirt: Rabbit
Kategorie: Antikörper
Spezies Reaktivität: Rat
Konjugation: Biotin-SP (long spacer)

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with both rat IgG and IgM. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.

 

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Klonalität: Polyclonal
Isotyp: Whole IgG
Puffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Reinheit: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Formulierung: Whole IgG
Antibody Type: Secondary Antibody
Application Verdünnung: 1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry