AffiniPure(TM) Sheep Anti-Mouse IgG (H+L)

Artikelnummer: JIM-515-005-003
Artikelname: AffiniPure(TM) Sheep Anti-Mouse IgG (H+L)
Artikelnummer: JIM-515-005-003
Hersteller Artikelnummer: 515-005-003
Alternativnummer: JIM-515-005-003
Hersteller: Jackson ImmunoResearch
Wirt: Sheep
Kategorie: Antikörper
Spezies Reaktivität: Mouse
Konjugation: Unconjugated

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

 

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Klonalität: Polyclonal
Isotyp: Whole IgG
Puffer: Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Preservative: None
Reinheit: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Formulierung: Sterile-filtered liquid
Lagerung: Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use. Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.
Antibody Type: Secondary Antibody
Application Verdünnung: 10-20 µg / ml Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.