Peroxidase-conjugated AffiniPure(TM) F(ab)2 Fragment Donkey Anti-Human IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Ms,Rb,Rat,Shp Sr Prot)

Produktübersicht

• Artikelname: Peroxidase-conjugated AffiniPure(TM) F(ab)2 Fragment Donkey Anti-Human IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Ms,Rb,Rat,Shp Sr Prot)
• Artikelnummer: JIM-709-036-149
• Hersteller Artikelnummer: 709-036-149
• Alternativnummer: JIM-709-036-149
• Hersteller: Jackson ImmunoResearch
• Wirt: Donkey
• Kategorie: Antikörper
• Spezies Reaktivität: Human
• Konjugation: HRP

Zusatzinformationen

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, mouse, rabbit, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

 

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Produkteigenschaften

• Klonalität: Polyclonal
• Isotyp: F(ab')₂ Fragment
• Puffer: Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free). Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish...
• Reinheit: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
• Formulierung: Freeze-dried solid
• Lagerung: Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended...
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: 1:500 - 1:5,000 for immunohisto/cytochemistry_x000D_ 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates_x000D_ 1:10,000 - 1:200,000 for Western blotting with ECL substrates_x000D_ Dilution factors are presented in the form...