Bacillus megaterium Expression Systems: The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale. B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall and the bacterium is able to secrete proteins into the growth medium. Protein yields are exceptionally high even if inexpensive substrates are used. Features: • Versatile protein production (intracellular and extracellular) • Ideal for small- and large-scale protein production • Inducible and constitutive expression systems available • No alkaline protease activity • High performance vectors • Vectors with two ribosome binding sites (2RBS) for simultaneous dual expression • Simple purification with different tags • Protoplasts specifically optimized for transformation • Endotoxin-free
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